TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1(Homo sapiens)
The Ohio State University
Curated by ChEMBL
The Ohio State University
Curated by ChEMBL
Affinity DataKd: 330nMAssay Description:Binding affinity to NHERF1 PDZ1 domain (unknown origin) expressed in Escherichia coli BL21 incubated for 1 hr by fluorescence polarization based comp...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [1-140](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 400nMpH: 6.4 T: 2°CAssay Description:Competition assays were performed in FP buffer (DTT was avoided for Cys-PTHRct-9) containing fixed concentrations of both the fluorescently labeled p...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [133-300](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 400nMpH: 6.4 T: 2°CAssay Description:Competition assays were performed in FP buffer (DTT was avoided for Cys-PTHRct-9) containing fixed concentrations of both the fluorescently labeled p...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [133-300](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 600nMpH: 7.4 T: 2°CAssay Description:Competition assays were performed in FP buffer (DTT was avoided for Cys-PTHRct-9) containing fixed concentrations of both the fluorescently labeled p...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [133-300](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 600nMpH: 7.0 T: 2°CAssay Description:Competition assays were performed in FP buffer (DTT was avoided for Cys-PTHRct-9) containing fixed concentrations of both the fluorescently labeled p...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1(Homo sapiens)
The Ohio State University
Curated by ChEMBL
The Ohio State University
Curated by ChEMBL
Affinity DataKd: 780nMAssay Description:Binding affinity to NHERF1 PDZ1 domain (unknown origin) expressed in Escherichia coli BL21 incubated for 1 hr by fluorescence polarization based comp...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [1-140](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 1.00E+3nMpH: 7.4 T: 2°CAssay Description:Competition assays were performed in FP buffer (DTT was avoided for Cys-PTHRct-9) containing fixed concentrations of both the fluorescently labeled p...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [1-140](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 1.00E+3nMpH: 7.0 T: 2°CAssay Description:Competition assays were performed in FP buffer (DTT was avoided for Cys-PTHRct-9) containing fixed concentrations of both the fluorescently labeled p...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1(Homo sapiens)
The Ohio State University
Curated by ChEMBL
The Ohio State University
Curated by ChEMBL
Affinity DataKd: 1.64E+3nMAssay Description:Binding affinity to NHERF1 PDZ1 domain (unknown origin) expressed in Escherichia coli BL21 incubated for 1 hr by fluorescence polarization based comp...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [133-300](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 2.40E+3nMpH: 7.4 T: 2°CAssay Description:Competition assays were performed in FP buffer (DTT was avoided for Cys-PTHRct-9) containing fixed concentrations of both the fluorescently labeled p...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1(Homo sapiens)
The Ohio State University
Curated by ChEMBL
The Ohio State University
Curated by ChEMBL
Affinity DataKd: 3.97E+3nMAssay Description:Binding affinity to NHERF1 PDZ1 domain (unknown origin) expressed in Escherichia coli BL21 incubated for 1 hr by fluorescence polarization based comp...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [1-140](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 4.70E+3nMpH: 7.4 T: 2°CAssay Description:Competition assays were performed in FP buffer (DTT was avoided for Cys-PTHRct-9) containing fixed concentrations of both the fluorescently labeled p...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [133-300](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 9.70E+3nMpH: 8.0 T: 2°CAssay Description:ITC measurements were conducted on a Microcal iTC200 (Malvern Instruments) in buffer consisting of 25 mM Tris (pH 8.0) and 100 mM NaCl. Proteins were...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [133-300](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 1.15E+4nMpH: 7.4 T: 2°CAssay Description:SPR measurements were performed with a Biacore X100 instrument (GE Healthcare Life Sciences, Piscataway, NJ) at 15.0 °C. The biotinylated PTHRct-...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [1-140](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 1.17E+4nMpH: 8.0 T: 2°CAssay Description:ITC measurements were conducted on a Microcal iTC200 (Malvern Instruments) in buffer consisting of 25 mM Tris (pH 8.0) and 100 mM NaCl. Proteins were...More data for this Ligand-Target Pair
TargetNa(+)/H(+) exchange regulatory cofactor NHE-RF1 [1-140](Homo sapiens (Human))
The University of Queensland
The University of Queensland
Affinity DataKd: 1.60E+4nMpH: 7.4 T: 2°CAssay Description:SPR measurements were performed with a Biacore X100 instrument (GE Healthcare Life Sciences, Piscataway, NJ) at 15.0 °C. The biotinylated PTHRct-...More data for this Ligand-Target Pair